ISOLATION, IDENTIFICATION, PARTIAL PURIFICATION, OPTIMIZATION AND CHARACTERIZATION OF ALKALINE PROTEASE FROM E. coli SHRUTI SHUKLA* AND O. P. VERMA

In the present study E. coli was used for the production of alkaline protease in eight different fermentation media. This bacterium was isolated from soil sample and screened for protease production. The enzyme production from E. coli was estimated using casein as substrate and its molecular weight was determined by SDS-PAGE. The enzyme activity was also studied at different parameters like pH, temperature, carbon source, nitrogen source, metal ions concentration and effect of inhibitors.